5’-Nucleotidase (5’-NT) - Diazyme Laboratories
The 5'-NT assay is based on the enzymatic hydrolysis of 5'-monophosphate (5'-IMP) to form inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H 2 O 2 ) by xanthine oxidase (XOD).













